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Intake of phenol-rich virgin olive oil improves the postprandialprothrombotic profile in hypercholesterolemic patients1–3 Juan Ruano, José Lo´pez-Miranda, Rafael de la Torre, Javier Delgado-Lista, Javier Ferna´ndez, Javier Caballero,María Isabel Covas, Yolanda Jiménez, Pablo Pérez-Martínez, Carmen Marín, Francisco Fuentes, andFrancisco Pérez-Jiménez ABSTRACT
concentrations have been linked to coronary heart disease (CHD) Background: Oxidative stress associated with postprandial lipemia
(2), and both can be regulated, at least partly, by alimentary contributes to endothelial dysfunction, which shifts hemostasis to a lipemia (3). Attention is currently focusing on investigating whether different components of the diet can regulate acute post- Objective: We investigated whether a high concentration of phenols
prandial changes in coagulation and fibrinolysis.
in olive oil can partly reverse this phenomenon.
Factor VII coagulant (FVIIc) has been linked to postprandial Design: Twenty-one hypercholesterolemic volunteers received 2
plasma triacylglycerol concentrations, which suggests an acute breakfasts rich in olive oils with different phenolic contents (80 or effect of triacylglycerol-rich lipoproteins (TRLs) on the activity 400 ppm) according to a randomized, sequential crossover design.
of FVII (FVIIa) (4). We previously reported that a Mediterranean Plasma concentrations of lipid fractions, factor VII antigen diet reduces fasting plasma concentrations of FVIIa in healthy (FVIIag), activated factor VII (FVIIa), and plasminogen activator men (5), a fact that might be related to the presence of olive oil in inhibitor-1 (PAI-1) activity were measured at baseline and post- this diet (6, 7). Williams (8) observed a reduction in postprandial FVIIa and factor VII antigen (FVIIag) plasma concentrations Results: Concentrations of FVIIa increased less (P ҃ 0.018) and
after the acute intake of an olive oil– based meal.
plasma PAI-1 activity decreased more (P ҃ 0.021) 2 h after the On the other hand, it has been suggested that PAI-1 activity high-phenol meal than after the low-phenol meal. FVIIa concentra- declines after the intake of meals rich in oleic acid as part of a tions 120 min after intake of the olive oil with a high phenol content Mediterranean-type diet, in both the postprandial (9) and fasting correlated positively with fasting plasma triacylglycerols (P ҃ states (10, 11). Virgin olive oil, which is the principal fat in this 0.001), area under the curve (AUC) of triacylglycerols (P ҃ 0.001), dietary pattern (12), contains both oleic acid and a wide range of and AUC of nonesterified fatty acids (P ҃ 0.024) and negatively micronutrients, among which phenolic compounds have dis- with hydroxytyrosol plasma concentrations at 60 min (P ҃ 0.039) played anti-thrombotic effects in cell culture and in vitro studies and fasting HDL-cholesterol concentrations (P ҃ 0.005). PAI-1 (13, 14). Studies in humans, however, are scarce, and more ev- positively correlated with homeostasis model assessment of insulin idence on these biological activities is needed (15). To further resistance (P ҃ 0.005) and fasting triacylglycerols (P ҃ 0.025) and investigate whether postprandial phenols from virgin olive oil inversely with adiponectin (P ҃ 0.026). In a multivariate analysis, affect hemostasis, we tested whether 2 breakfasts rich in this oil, the AUCs of nonesterified fatty acids (R2 ҃ 0.467; ␤: 0.787; SE:0.02; P  0.001) and adiponectin (R2 ҃ 0.232; ␤: Ҁ1.594; SE: 1 From the Lipids and Atherosclerosis Research Unit, Reina Sofia Uni- 0.629; P  0.05) were the strongest predictors of plasma FVIIa and versity Hospital, University of Cordoba, Ciber Fisiopatología Obesidad y Nutricio´n (CB06/03), Instituto de Salud Carlos III, Spain (JR, JL-M, JD-L, Conclusions: A virgin olive oil with a high content of phenolic
YJ, PP-M, CM, FF, and FP-J); the Pharmacology Research Unit (RdlT) and compounds changes the postprandial hemostatic profile to a less the Lipids and Epidemiology Cardiovascular Research Unit (MIC), Institut Am J Clin Nutr 2007;86:341– 6.
Municipal d’Investigacio Medica (IMIM), Barcelona, Spain; the Departmentof Plant Biology, Faculty of Biology, University of Cordoba, Cordoba, Spain KEY WORDS
Olive oil, polyphenols, postprandial lipemia, (JF); and the Biochemical Laboratory, Reina Sofia University Hospital, plasminogen activator inhibitor-1, PAI-1, activated factor VII, Supported in part by research grants from the Spanish Plan for RѿD (SAF 03/05770 to FP-J), Plan Andaluz de Investigacio´n (Consejeria deInnovacio´n, Proyectos de Investigacio´n de Excelencia AGR 05/00922 toFP-J), and the “Reina Sofía-Cajasur” Cultural Foundation. JR and JD-L are INTRODUCTION
currently engaged in a Research Specialization Program for the Spanish Endothelial dysfunction is one of the first steps in the devel- 3 Address reprint requests to F Pérez-Jiménez, Lipids and Atherosclerosis opment of arteriosclerosis, and it is characterized by a thrombo- Research Unit, Reina Sofia University Hospital, Avenue Menéndez Pidal, genic state caused by an imbalance between procoagulant and s/n, 14004 Cordoba, Spain. E-mail: [email protected].
profibrinolytic activity (1). Among the procoagulant factors, plasminogen activator inhibitor-1 (PAI-1) and factor VII (FVII) Accepted for publication April 4, 2007.
Am J Clin Nutr 2007;86:341– 6. Printed in USA. 2007 American Society for Nutrition but with different contents of phenolic compounds, had different and were subsequently analyzed in a Jasco FP-920 spectroflu- effects on hemostasis postprandially.
orometer (Jasco, Tokyo, Japan) at an excitation wavelength of294 nm and an emission wavelength of 340 nm. A spectropho- SUBJECTS AND METHODS
tometer (UNICAM 5625, Cambridge, UK) was used to measuretotal carotenoid (670 nm) and chlorophyll (472 nm) contents. No Subjects
significant differences in any of the micronutrient concentrations Twenty-one hypercholesterolemic subjects (5 men and 16 were found, except for the polyphenol fraction.
women) aged 53 to 68 y and with a mean body mass index (in Plasma samples
kg/m2) of 25.4 Ȁ 4.1 (range: 23.5–27.1) participated in the study.
All were patients who were being followed up in the Lipids and Samples from the fasting and postprandial states were col- Atherosclerosis Unit at the Reina Sofía University Hospital in lected in tubes containing 1 g EDTA/L or 3.8% citrate and were Cordoba. Plasma total cholesterol concentrations were between stored in containers with ice and kept in the dark. Special care was 200 and 350 mg/dL and plasma triacylglycerol concentrations taken to avoid exposure to air, light, and ambient temperature.
were 200 mg/dL. The women were postmenopausal (but were Plasma was separated from whole blood by low-speed centrifu- not undergoing hormone replacement therapy). None of the par- gation at 1500 ҂ g for 15 min at 4 °C within 1 h of extraction.
ticipants showed evidence of chronic diseases, high alcohol con- Plasma polyphenol concentrations
sumption, or family history of early-onset cardiovascular dis-ease. None of the subjects were active smokers. The study was Concentrations of tyrosol, hydroxytyrosol, and 3-O-methyl- approved by the Human Investigation Review Committee at the hydroxytyrosol (MHT), a biological metabolite of hydroxyty- Reina Sofia University Hospital. All the participants gave their rosol, were measured by gas chromatography–mass spectrome- informed consent before joining the study.
try in plasma samples at 0 and 60 min (16, 17).
Experimental design
Lipid analysis and biochemical determinations
The participants were instructed to not take vitamins, soya Concentrations of the different lipid variables were analyzed supplements, or any drug treatments, including hormone treat- with a modular autoanalyzer (DDPPII Hitachi; Roche, Basel, ment, for the 6 wk preceding the study. Three patients were Switzerland) with the use of Boehringer-Mannheim reagents.
taking atorvastatin, 10 mg/d, which was discontinued 6 wk be- Concentrations of total cholesterol and triacylglycerol were mea- fore the randomization step. The subjects were shown how to sured by colorimetric enzymatic methods (18, 19). HDL- follow a low-fat, carbohydrate-rich diet during that period to cholesterol concentrations were measured by colorimetric assay eliminate potential differences in their usual dietary habits. Com- after the lipoproteins containing apolipoprotein (apo) B were pliance with the stabilization diet was assessed after 2 and 4 wk precipitated with polyethylene glycol (20). LDL-cholesterol by means of a 3-d record and a food-frequency dietary question- concentrations were estimated by using the Friedewald formula naire. The participants were instructed to avoid consuming on the basis of total cholesterol, triacylglycerol, and HDL- polyphenol-rich foods (such as fruit or juices, wine, grape juice, cholesterol values (21). Apo A-I and apo B concentrations were chocolate, coffee, tea, olive oil, or soya) or performing intense measured by immunoturbidimetry (22). The chylomicron and physical exercise in the 24 h before the experimental breakfast.
large VLDL fractions of TRLs were isolated from 4 mL plasma The following morning they came to the hospital after fasting for overlayered with 0.15 mol NaCl/L, 1 mmol EDTA/L (pH 7.4; 12 h. By use of a randomized, sequential crossover design, the density: 1.006 kg/L) by a single ultracentrifugal spin (28 000 ҂ participants were given 1 of 2 breakfasts consisting of 60 g white g, 30 min, 4 °C) in a type 50 rotor (Beckman Instruments, Ful- bread, 40 mL virgin olive oil (Carapelli Firenze SpA, Florence, lerton, CA). Chylomicrons contained in the top layer were re- Italy) with either a high (A, 400 ppm) or a low (B, 80 ppm) moved by aspiration after the tubes were cut. The infranatant content of phenolic compounds and 60 000 IUs vitamin A/m2 fraction was centrifuged at a density of 1.019 kg/L for 24 h at body surface area. Patients starting with the A type breakfast 115 000 ҂ g in the same rotor. The nonchylomicron fraction of consumed the B type after 1 wk, and conversely. Olive oil B was TRL (also referred to as small TRL) was removed from the top of obtained by the extraction of most of the phenolic compounds in the tube. All operations were performed in subdued light. Large olive oil A, so that both oils had similar contents of their remain- and small TRL fractions were kept at Ҁ70 °C until total choles- ing macro- and micronutrients. The procedure involved washing terol and triacylglycerol concentrations were analyzed. Fasting olive oil A in a separation funnel with an equal quantity of plasma adiponectin and resistin were measured by enzyme- double-distilled water. The mixture was shaken for 3 min and left linked immunosorbent assay with the Quantikine Human Adi- to settle to facilitate the separation process. The aqueous phase ponectin/Acrp30 Immunoassay and Quantikine Human Resistin was then discarded and the procedure repeated. The concentra- Immunoassay (R&D Systems Inc, Minneapolis, MN). Plasma tion of phenolic compounds was measured until it fell to trace glucose concentrations were measured with a Hitachi 917 ana- lyzer (Boehringer Mannheim, Mannheim, Germany) by the glu- Throughout the 4-h duration of the study, the subjects neither cose oxidase method (GOD-PAP). Plasma insulin concentra- performed physical activity nor consumed anything but water.
tions were measured by microparticle enzyme immunoassay Venous blood was drawn at 0, 30, 60, 120, and 240 min after (Abbott Diagnostics, Matsudo-shi, Japan). Nonesterified fatty acid concentrations were measured by enzymatic colorimetricassay (Roche Diagnostics, Penzberg, Germany). The homeosta- Composition of the olive oils
sis model assessment of insulin resistance (HOMA-IR) was de- Tocopherols were measured by separating the different to- fined by the validated definition: HOMA-IR ҃ [fasting glucose copherol isomers by means of HPLC (Beckman, Palo Alto, CA) (mmol/L) ҂ fasting insulin (␮U/mL)/22.5] (23).
PHENOLS REDUCE PROTHROMBOSIS POSTPRANDIALLY performed. A value of P  0.05 was considered to be statistically Baseline characteristics of the hypercholesterolemic participants in the significant. All analyses were carried out by using the statistical software package SPSS (version 11.0; SPSS Inc, Chicago, IL).
Basal and postprandial metabolic parameters
The clinical characteristics of the participants at baseline are shown in Table 1. No significant differences were observed in
any of the incremental AUCs of the main metabolic variables after the intake of either of the olive oils (Table 2).
Basal and postprandial concentrations of tyrosol,
hydroxytyrosol, and MHT
1 All values are x៮ Ȁ SD (range in parentheses); n ҃ 21. NEFA, non- We observed a greater increase in concentrations of plasma esterified fatty acids; HOMA-IR, homeostasis model assessment of insulin tyrosol, hydroxytyrosol, and MHT after the intake of the olive oil with a high phenolic compound content than after the olive oil
with a low content of phenols (Figure 1).
Measurement of FVIIag, FVIIa, and PAI-1
We measured concentrations of PAI-1 in the frozen plasma FVIIag, FVIIa, and PAI-1
samples by means of a tissue-type plasminogen activator– based Postprandial plasma concentrations of FVIIag and FVIIa and immunoactivity assay (Chromolize PAI-1; Trinity Biotech, PAI-1 activity are presented in Figure 2. The intake of both olive
County Wicklow, Ireland) and concentrations of FVIIag (Asse- oils significantly increased FVIIa activity (P ҃ 0.002) and de- rachrom VII:Ag; Diagnostica Stago, Asnières sur Seine, France) creased FVIIag (P ҃ 0.050) and PAI-1 activity (P ҃ 0.001) with and FVIIa (Imubind; American Diagnostica Inc, Greenwich, respect to baseline concentrations, which indicates a change in CT) by enzyme-linked immunosorbent assay. All measurements these variables during postprandial lipemia. Analysis of the in- teraction between diet and time showed a smaller increase in FVIIa concentration (P ҃ 0.050) and a greater decrease in PAI-1 Statistical analysis
activity (P ҃ 0.047) after the phenol-rich breakfast than after the Duplicate values from each subject were calculated before data analysis. Comparisons among the end values for each treat- When we compared percentage changes from baseline values ment period were made after adjustment for baseline values. The in FVIIag, FVIIa, and PAI-1 values at 120 min, we found a percentage change between concentrations at the beginning of smaller postprandial increase in FVIIa plasma concentrations each breakfast (basal) and concentrations 60 and 120 min after (39.7 Ȁ 30.8% compared with 20.3 Ȁ 26.5%; P ҃ 0.018) and a intervention with the olive oils was calculated. The area under the greater decrease in PAI-1 plasma activity (Ҁ29.1 Ȁ 32.8% com- curve (AUC) was defined as the area between the plasma con- pared with Ҁ52.8 Ȁ 30.4%; P ҃ 0.021), but no significant centration–versus–time curve and a line drawn parallel to the differences in FVIIag, after the phenol-rich olive oil than after the horizontal axis through the 0 h concentration. These areas were calculated by a computer program using the trapezoidalrule. All data presented in the text and tables are expressed as Regression analysis
means Ȁ SDs. The normality of variables was assessed by the To further evaluate predictors of FVIIa and PAI-1, we per- Kolmogorov-Smirnov test. The data were analyzed by analysis formed a correlation analysis between these 2 variables and body of variance (ANOVA) for repeated measures. Diet, time, and mass index (BMI), metabolic variables, HOMA-IR, adiponectin, their interaction were included in the model in addition to fasting resistin, and plasma phenol concentrations. In a univariate cor- concentrations as a covariate. Pearson’s linear correlation coeffi- relation analysis, FVIIa concentrations correlated positively cient was calculated, and a multiple linear regression analysis was with triacylglycerol (r ҃ 0.503, P ҃ 0.001), the AUC of plasma TABLE 2
Areas under the plasma postprandial response curves (AUCs) after the intake of the low-phenol and the phenol-rich olive oil (OO)-based breakfasts1
1 All values are x៮ Ȁ SD; n ҃ 21. NEFAs, nonesterified fatty acids; TG, triacylglycerols; TRLs, triacylglycerol-rich lipoproteins.
2 One-factor ANOVA.
FIGURE 1. Mean (ȀSD) concentrations of tyrosol, hydroxytyrosol, and 3-O-methyl-hydroxytyrosol (MHT) at baseline and 60 min after intake of a
phenol-rich olive oil breakfast (400 ppm; f) or a low-phenol olive oil breakfast (80 ppm; Ⅺ). n ҃ 21. For each panel, bars with different lowercase letters aresignificantly different from each other, P  0.05. P1: diet effect; P2: time effect; P3: diet ҂ time interaction. ANOVA for repeated-measures. Baseline valueswere used as covariants.
triacylglycerol (0.567, P ҃ 0.016), and the AUC of nonesterified triacylglycerol-rich lipoproteins by lipoprotein lipase may be an fatty acids (r ҃ 0.234, P ҃ 0.024) and negatively with concen- important source of elevated concentrations of fatty acid anions trations of hydroxytyrosol (r ҃ Ҁ0.465, P ҃ 0.039) and HDL near the endothelium. These fatty acids are substrates for the cholesterol (r ҃ Ҁ0.593, P ҃ 0.005). Strong direct relations were lipoperoxidation produced by the increase in oxidative stress found between PAI-1 activity and HOMA-IR (r ҃ 0.602, P ҃ during the postprandial period. Olive oil phenolic compounds 0.005) and triacylglycerol (r ҃ 0.487, P ҃ 0.025). PAI-1 activity have been shown to act as chain-breaking antioxidants for the correlated negatively with fasting adiponectin concentrations autocatalytic chain reaction of fatty acid peroxidation (26). The (r ҃ Ҁ0.485, P ҃ 0.026).
attenuating effect of olive oil–rich test meals on FVII was shown To explore the importance of potential predictors, 2 stepwise previously (6, 27), but these studies focused on the effect of multiple linear regression models were fitted for FVIIa and modified test meal dietary fat composition. What proportion of PAI-1. After adjustment for HOMA-IR, fasting triacylglycerol, this effect is due to the phenolic or fatty acid profile of virgin olive and the AUC for triacylglycerol, only the incremental AUC of nonesterified fatty acids remained significantly associated with In the present study, we found a significant decrease in post- FVIIa in the model (R2 ҃ 0.467; ␤: 0.787; SE: 0.02; P  0.001).
prandial concentrations of FVIIag, but no significant differences A similar strategy was used to assess the predictors of plasma in the effects of these 2 types of olive oil. At the same time, there PAI-1 concentrations. After adjustment for HOMA-IR, BMI, was a smaller increase in FVIIa after the ingestion of an olive oil and triacylglycerol, plasma adiponectin concentrations remained with a high content of phenols than after the olive oil with a low significantly associated with plasma PAI-1 (R2 ҃ 0.232; ␤: content of these compounds. Because the sole difference in the 1.594; SE: 0.629; P  0.05).
composition of these 2 oils was their phenolic content, these datasuggest that the effect of the diet on the decrease in FVIIag is due DISCUSSION
to the difference in their fat content, and the effect on FVII Our study showed that, in patients with hypercholesterolemia, activation to their content of phenols.
the consumption of a breakfast containing virgin olive oil with a How phenols interfere with this postprandial activation of high content of phenols induces a smaller postprandial increase FVIIa is not known. We previously showed the protective effect in the concentration of FVIIa and a greater decrease in PAI-1 of olive oil phenols on the postprandial microvascular endothe- plasma activity than the same olive oil with a low content of lial function of hypercholesterolemic persons (28), an effect that phenols. It has been suggested that the postprandial hypertriglyc- is strongly influenced by procoagulant and prooxidant factors. It eridemia that follows the intake of high-fat meals activates FVII.
could be hypothesized that the known antioxidant properties of The intrinsic mechanism of this activation is not clear, although phenols act as a potent buffer in the vicinity of endothelial cells, it is known that some of the reactions for activation of hemostatic thus reducing the activation of FVIIa.
factors are due to exposure to lipid bilayers with negative We also observed a significant reduction in PAI-1 concentra- charges, such as those of denuded endothelium or the surface of tions 120 min after the ingestion of olive oil with a high phenolic platelets or oxidized LDL (24). Some studies have observed that content. Although changes in fasting PAI-1 concentrations after FVIIa plasma concentrations correlate positively with plasma prolonged dietary intervention periods have been described in phospholipid concentrations (25). Our study showed a signifi- several studies, less evidence exists of this effect of the diet in the cant association between the incremental AUC of nonesterified postprandial state. Furthermore, it is difficult to make a global fatty acids and postprandial changes in FVIIa. The hydrolysis of interpretation of the postprandial effects on PAI-1 on the basis of PHENOLS REDUCE PROTHROMBOSIS POSTPRANDIALLY between HOMA-IR, adiponectin, and PAI-1 were previouslyreported in both adults (40 – 42) and children (43). PAI-1 con-centrations are directly influenced by insulin (44), even in situ-ations of insulin resistance (45), a fact that has been explained interms of abdominal fat, via a higher concentration of cytokines insubjects with central obesity (41). Nevertheless, the associationof PAI-1 and insulin resistance has also been found in nonobesecohorts (46). These apparently contradictory findings may bepartially explained by the identification of a regulatory element(AP-1 response element) in the PAI-1 promoter. This elementenhances PAI-1 transcription by a factor of 7 when stimulated byinsulin increases mediated by FOX protein transcription factors(47), which may explain the association between insulin resis-tance and PAI-1. Interestingly, the same element also induces a3-fold rise in the PAI-1 transcription rate in the presence ofoxidative stress (48), as occurs in the postprandial state. Thedouble regulation of the PAI-1 promoter element may explainboth the fasting correlations between adiponectin, HOMA-IR,and PAI-1 and the larger postprandial decrease in PAI-1 after thephenol-rich breakfast as a result of its antioxidant properties and thelower activation of nuclear factor ␬B that occurs after meals rich invirgin olive oil (49). In line with our findings, Pacheco et al (15) recently found an enhancement in postprandial hemostasis after avery phenol-rich olive oil meal (1125 ppm of phenolic compounds),an enhancement that they evaluated in terms of a greater decrease inPAI-1, smaller tissue factor and fibrinogen AUCs, and a largerpostprandial decrease in tissue-type plasminogen activator.
The results of the present study may partly explain earlier contradictory results of studies that tested the effects of olive oilon hemostasis. It is possible that the concentrations of micro-components of the olive oil used in some of those studies did notreach the levels needed to activate the antithrombotic properties of olive oil. However, and although this study deals with themicrocomponents of virgin olive oil, we should still think interms of evaluating the biological properties of complete foods.
In this perspective, our findings provide new evidence of thehealthy effects of virgin olive oil. In conclusion, the consumptionof a breakfast containing olive oil rich in phenolic compoundsmay improve the thrombogenic postprandial profile of FVIIa andPAI-1 concentrations associated with acute fat intake.
FIGURE 2. Mean (ȀSD) acute effect on postprandial plasma factor VII
antigen (FVIIag), activated factor VII (FVIIa), and plasminogen activator The authors express their gratitude to the “Instituto Nutrizionale Car- inhibitor-1 (PAI-1) activity after the ingestion of a phenol-rich olive oil apelli” (Florence, Italy), to the CEAS (Centro de Excelencia Investigadora breakfast (400 ppm; f) or a low-phenol olive oil breakfast (80 ppm; Ⅺ). P1: del Accite de oliva y la Salud), Jae´n, Spain; and to all of the subjects for their diet effect; P2: time effect; P3: diet ҂ time interaction. ANOVA for repeated- measures. Baseline values were used as covariants.
The contributions of the authors were as follows—JR, JL-M, and FP-J: responsible for the conception and design of the study; JR, FF, CM, and YJ: these studies because of the different designs and methods used.
responsible for the provision of study materials or subjects; JR, JL-M, JC, JF, Both increases (3, 29) and reductions (9, 30, 31) in PAI-1 con- FF, RdlT, MIC, and CM: responsible for the collection and assembly of data; centrations after meals with a high fat content have been de- JR, JL-M, JC, JF, YJ, and FP-J: responsible for the analysis and interpretation scribed. It has even been suggested that these data might simply of the data; JL-M, CM, FF, and FP-J: provided statistical expertise; JR, PP-M,JL-M, and FP-J: responsible for drafting the manuscript; JD-L, RdlT, MIC, be the result of a circadian variation in PAI-1 concentrations, JL-M, and FP-J: responsible for the critical review of the manuscript and for without any direct relation to diet (32, 33). Nor is there a clear important intellectual content; FP-J: obtained funding. None of the authors explanation of the mechanism by which PAI-1 concentrations had any personal or financial conflicts of interest.
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If you are taking any medications on this list, they should be discontinued 14 days prior to surgery and only Tylenol should be taken for pain. All other medications that you are currently taking must be specifically cleared by your doctor prior to surgery. It is absolutely necessary that all of your current medications be specifically cleared by your doctor and the nursing staff. Medicati

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