Microsoft word - microbiology_complete.doc
BL21(DE3)
F–
ompT gal dcm lon hsdSB(
rB-
mB-) λ(DE3)
- an
E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and
lacIq - Transformed plasmids containing T7 promoter driven expression are repressed until IPTG
induction of T7 RNA polymerase from a lac promoter.
BL21 (DE3) pLysS
F-
ompT gal dcm lon hsdSB(
rB-
mB-) λ(DE3) p
LysS(cmR)
- pLys plasmid chloramphenicol resistant; grow with chloramphenicol to retain plasmid
- Chloramphenicol resistant
- The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymerase which
reduces and almost eliminates expression from transformed T7 promoter containing plasmids
when not induced.
DB3.1
F-
gyrA462 endA1 glnV44 ∆(
sr1-
recA)
mcrB mrr hsdS20(
rB-,
mB-)
ara14 galK2 lacY1 proA2 rpsL20 xyl5 ∆
leu mtl1 - useful for propagating plasmids containing the ccdB gene (
gyrA462 ).
- streptomycin resistant
DH5α
F-
endA1 glnV44 thi-1
recA1 relA1 gyrA96 deoR nupG Φ80
lacZ∆
M15 ∆(
lacZYA-
argF)U169,
hsdR17(rK- mK+), λ–
- nalidixic acid resistant
DH10B (Invitrogen)
F-
endA1 recA1 galU galK deoR nupG rpsL ∆
lacX74 Φ80
lacZ∆M15
araD139 ∆(
ara,
leu)7697
mcrA ∆(
mrr-hsdRMS-mcrBC) λ-
- suitable for cloning methylated cytosine or adenine containing DNA
- Streptomycin resistant
JM109
endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ ∆(
lac-
proAB)
glnV44 e14- [F'
traD36 proAB+
lacIq lacZ∆
M15]
hsdR17(
rK-
mK+)
- Partly restriction-deficient; good strain for cloning repetitive DNA (RecA–).
- nalidixic acid resistant
SURE (Stratagene)
endA1 glnV44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5
uvrC e14- ∆(
mcrCB-
hsdSMR-mrr)171 F'[
proAB+
lacIq lacZ∆
M15 Tn10]
- increased stability for inverted repeats and Z-DNA
- nalidixic acid resistant
- kanamycin resistant
- tetracycline resistant
SURE2 (Stratagene)
endA1 glnV44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5
uvrC e14- ∆(
mcrCB-
hsdSMR-
mrr)171 F'[
proAB+
lacIq lacZ∆
M15 Tn10
Amy CmR]
- increased stability for inverted repeats and Z-DNA
- nalidixic acid resistant
- kanamycin resistant
- tetracycline resistant
- chloramphenicol resistant for < 40 µg/ml, sensitive for > 100 µg/ml
XL1-Blue (Stratagene)
endA1 gyrA96 thi-1 recA1 relA1 lac glnV44 F'[ ::
Tn10
proAB+
lacIq ∆
lacZM15]
hsdR17(
rK-
mK+)
- nalidixic acid resistant
- tetracycline resistant
SW102
F-
endA1 recA1 ∆
galK deoR nupG rpsL ∆
lacX74 Φ80
lacZ∆M15
araD139 ∆(
ara,
leu)7697
mcrA ∆(
mrr-hsdRMS-mcrBC) [ λ
cI857 (
cro-bioA) <>]
- Streptomycin resistant
Source: http://francois.schweisguth.free.fr/protocols/Bacterial_strains.pdf
Dr. med. Alexander Walz Zelgmatt 5 CH-8132 Egg Switzerland Dr. Alexander Walz Zelgmatt 5 CH-8132 Egg Vasella/Novartis ./. Kessler/VGT In Ergänzung zur Stellungnahme vom 28.01.2010 Zum Vorwurf, es handele sich bei meiner Stellungnahme um ein reines Parteigutachten, welchem keinerlei Beweiswert zukäme, möchte ich Folgendes an Eides statt erklären: 1. Habe ich Herrn Dr. Kessler weder je
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