Microsoft word - 2011 04 26 mgc trg programma v2ves.doc
MGC-AIO course: Transgenesis, Gene Targeting and in vivo Imaging Leiden, LUMC, A: Basic course Building 1, lecture hall 2 (K1-S) May 9-11, 2011 B: International Innovative Mouse Model workshop Building 3, Buruma hall May 12-13, 2011
Organizers: Sjef Verbeek, Els Robanus Maandag and Riccardo FoddeProgram and Additional Information Monday, May 9 Lecture hall 2 (K1-S) N.B. Lunches will be provided throughout the week to all participants free of charge Tea / Coffee will be provided between 3-4 p.m. Afternoon Lecture hall 2 (K1-S) Tuesday, May 10 Lecture hall 2 (K1-S)
Parallel sessions in vivo imaging
Afternoon Lecture hall 2 (K1-S) Wednesday, May 11 Morning: Lecture hall 2 (K1-S) Afternoon: Lecture hall 2 (K1-S)
Discussion forum: Which animal model is the best?
6th Workshop on Innovative Mouse Models Leiden University Medical Center, Leiden, the Netherlands Topics rAAV directed gene targeting, recombineering, Zinc-finger nuclease assisted gene targeting, Epigenetic programming in iPS cells, ES cell derivation and culture, Meganuclases, TALE nucleases, Invited speakers Tomas Cermak Engineered TAL effector nucleases: new tools for genome Ivo Huijbers Fast track production of complex tumor models starting from GEMM-derived embryonic stem cells Rob Howes rAAV mediated gene targeting in human and animal cells
Cambridge Samer Hussein Genetic mosaicism and selection during reprogramming to pluripotency
Ralf Kühn Zinc finger nuclease assisted gene targeting Munchen Guillermo Montoya Molecular scissors for in situ cellular repair
Madrid Jennifer Nichols Manipulating pathways to improve ES cell culture and derivation Francis Stewart Advanced transgenesis and genome engineering using recombineering and site-specific recombinases Suggestions for further reading
Size matters: use of YACs, BACs and PACs in transgenic animals (Review)
Patricia Giraldo & Lluís Montoliu; Transgenic Res 10, 83–103, 2001.
Generating transgenic mice from bacterial artificial chromosomes: transgenesis efficiency, integration
Margaret L. Van Keuren et al .; Transgenic Res (2009) 18, 769–785
Transposon-mediated genome manipulation in vertebrates (Review)
Zoltán Ivics et al.; Nature Meth (2009) 6, 415-422
The impact of site-specific recombinases on genetic analyses in mice (Review)
Catherine S. Branda and Susan M. Dymecki; Dev Cell (2004), 6, 7–28,
Engineering chromosomal rearrangements in mice (Review)
Yuejin Yu and Allan Bradley; Nat Rev Genet (2001), 2, 780-790
Contemporary approaches for modifying the mouse genome (Review)
David J. Adams and Louise van der Weyden; Physiol Genomics (2008), 34, 225-238
The mouse ascending: perspectives for human-disease models (Review)
Nadia Rosenthal and Steve Brown; Nat Cell Biol (2007), 9, 993-999
Non-germline genetically engineered mouse models for translational cancer research (Review)
Joerg Heyer et al.; Nat Rev Cancer (2010), 10, 470-480
F0 generation mice fully derived from gene-targeted embryonic stem cells allowing immediate
William T Poueymirou, et al ; Nat Biotech (2007), 25, (1), 91-99
A mouse knockout library for secreted and transmembrane proteins
Tracy Tang et al., Nat Biotech (2010), 28, 749-755
Toward simpler and faster genome-wide mutagenesis in mice
Sen Wu1 et al., Nat Genet (2007), 39, 922-930
Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with
Marina Gertsensteinet al.; PLoS ONE (2011), 5 (6), e11260
A practical summary of site-specific recombination, conditional mutagenesis, and tamoxifen induction
Anastassiadis K et al.; Methods Enzymol (2010), 477,109-23
Imaging in the era of molecular oncology (Review)
Ralph Weissleder & Mikael J. Pittet Nature (2008), 452, 580-589
Nagy, A.; Gersenstein, M.; Vintersten, K.; Behringer R.,
Cold Spring Harbor Laboratory Press 2002
Practical Approach Oxford University Press 2006
Mammalian and Avian Transgenesis New Approaches
WEBSITES OF RESOURCES A. International Knockout Mouse Consortia
(Cell 2007; 128: 9-13 and Cell (2007), 129: 235)
http://www.komp.org/
EUCOMM (Europe): http://www.eucomm.org/
NorCOMM (Canada): http://www.norcomm.org/index.htm http://www.tigm.org/
Portal: The International Knockout Mouse Consortium (IKMC):
http://www.knockoutmouse.org/about resources
International Mouse Strain Resources (IMSR): global searchable catalog of mouse strain resources
http://www.findmice.org/index.jsp
JAX Mice (The Jackson Laboratory): mouse product list, technical support, mice:
http://jaxmice.jax.org/index.html
Induced Mutant Resource (IMR): genetically engineered mice, The Jackson Laboratory
http://www.jax.org/imr/index.html
Mutant Mouse Regional Resource Centers (MMRRC): mouse strain and ordering information from
NCRR's regional mutant mouse repositories: http://www.mmrrc.org/
Mammalian Genetics Unit (MGU): mouse stock list, mouse Embryo Bank, MRC, Harwell (UK)
http://www.har.mrc.ac.uk/
European Mouse Mutant Archive (EMMA): repository for transgenic mouse lines (Italy):
http://www.emmanet.org/
Oak Ridge National Laboratory (ORNL): mouse mutant stocks, Oak Ridge National Laboratory
http://www.nih.gov/science/models/mouse/resources/ornl.html
Neuromice.org: database and repository of mouse neurological mutants: http://www.neuromice.org/
Wellcome Trust Sanger Institute Cambridge UK (MICER)
http://www.sanger.ac.uk/resources/mouse/ C. Mouse and expression data bases
Mouse Atlas of Gene Expression (Canada):
http://www.mouseatlas.org/data/mouse/project_tissue_view http://www.brain-map.org/
Gene Expression Database (GXD) (MGI): http://www.informatics.jax.org/expression.shtml D. Anatomy
The Anatomy of the Laboratory Mouse Margaret J. Cook
http://www.informatics.jax.org/cookbook/ E. Cre transgenic mouse strains
The CREATE (Coordination of resources for conditional expression of mutated mouse alleles) project
http://www.creline.org.
Cre-X-Mice: a database of Cre transgenic lines
http://nagy.mshri.on.ca/cre_new/index.php F. General http://www.ensembl.org/Mus_musculus/Info/Index/
The “International Society for Transgenic Technologies (ISTT)” is a non-profitmaking Society
http://www.transtechsociety.org/ WHO IS WHO Faculties Organizers/ Lecturers Prof. Riccardo Fodde Experimental Pathology, Erasmus MC, Rotterdam. Tel.: 010-4088490 E-mail: [email protected] Dr. Els Robanus Maandag Dept. of Human and Clinical Genetics, LUMC, Leiden Tel.: 071-5269511 E-mail: [email protected] Dr. Sjef Verbeek Dept. of Human and Clinical Genetics, LUMC, Leiden Tel.: 071-5269410 E-mail: [email protected] Lecturers Cor Breukel Dept. of Human and Clinical Genetics, LUMC, Leiden Tel.: 071-5269414 E-mail: [email protected] Dr. Lee Fradkin Dept. of Molecular Cell Biology, LUMC, Leiden Tel: 071-5269228 E-mail: [email protected] Prof. Jeroen den Hertog Hubrecht Institute, NIOB KNAW, Utrecht Tel: +31 30 2121800 E-mail: [email protected] Dr. Jos Jonkers Div. of Molecular Biology, NKI, Amsterdam Tel.: 020-5122000 E-mail: [email protected] Dr. Eric Kaijzel Dept. of Endocrinology and Metabolic Diseases, LUMC, Leiden Tel.: 071-5263076 E-mail: [email protected] Dr. Rik Korswagen Hubrecht Institute, NIOB KNAW, Utrecht Tel.: 030-2121981 E-mail: [email protected] Dr. Nicolas Legrand AIMM Therapeutics, Amsterdam Tel.: 020-5666488 E-mail: [email protected] Prof. Hein te Riele Div. of Molecular Biology, NKI, Amsterdam Tel.: 020-5122084 E-mail: [email protected] Dr. Catherine Robin Dept. of Cell Biology, Erasmus MC, Rotterdam Tel.: 010-7044795 E-mail: [email protected] Dr. Daniela Salvatori Animal Facility (PDC), LUMC, Leiden Tel.: 071-5269688 E-mail: [email protected] Dr. Margriet Snoek Div. of Molecular Genetics, NKI, Amsterdam Tel.: 020-5122001 E-mail: [email protected] Dr. Marcel Veltrop Dept. of Human and Clinical Genetics, LUMC, Leiden Tel.: 071-5269427 E-mail: [email protected] Prof. Arn van den Maagdenberg Dept. of Human and Clinical Genetics, LUMC, Leiden Tel.: 071-5269460 E-mail: [email protected] Dr. Harald Mikkers Dept. of Molecular Cell Biology, LUMC, Leiden Tel: 071-5269668 E-mail: [email protected] Dr. Marc van de Wetering Hubrecht Institute, NIOB KNAW, Utrecht Tel.: 030-2121848 E-mail: [email protected] Dr. Joop Wiegant Dept. of Molecular Cell Biology, LUMC, Leiden Tel: 071-5269222 E-mail: [email protected]
PRACTICE GUIDELINE RECOMMENDED DRUGS FOR EMERGENCY CENTRES This Practice Guideline sets out the recommended drugs to be held in an Emergency Centre. The following emergency drugs and IV solutions are listed as essential (must all be available, together with protocols addressing their use) and recommended (EMSSA recommends that the Emergency Centre holds these drugs, along with pr
Data Exclusivity Periods and Next Generation Improvements to Innovator Biologics: Key Issues Duke University Department of Economics Working Paper, No. 2009-05 April 29, 2009 Authors: Henry Grabowski Professor of Economics and Director of the Program in Pharmaceuticals and Health Economics Duke University [email protected] Iain Cockburn Professor of Finance and Economics Bost
MGC-AIO course: 
6th Workshop on