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TECHNICAL PRODUCT INFORMATION Brilliant Green Agar Brilliant Green Agar w/Novobiocin (15 mcg/ml) Brilliant Green Agar w/Nalidixic Acid (50 mcg/ml) Brilliant Green Agar w/Nalidixic Acid (100 mcg/ml) & Novobiocin (15 mcg/ml) Brilliant Green Agar w/Sulfadiazine (80 mcg/ml) INTENDED USE: Brilliant Green Agar is a selective medium for the isolation of Salmonella species other than S. typhi and S. paratyphi A from fecal specimens, dairy products, or other material. Brilliant Green Agars w/Sulfadiazine, Nalidixic Acid and/or Nalidixic Acid & Novobiocin are used in food testing for Salmonella especially in eggs and poultry products1, 4. HISTORY/SUMMARY: Kristensen, Lester and Jurens2 first described Brilliant Green Agar for use as a primary plating medium for the isolation of Salmonella. Kauffman3 later modified the formula and found increased isolation of Salmonella from fecal material when specimens were first grown in Tetrathionate Broth with subsequent subculture onto the modified Brilliant Green Agar. It is recommended that Brilliant Green Agar be used in parallel with other, less inhibitory media (i.e.: Xylose Lysine Deoxycholate Agar, Hektoen Enteric Agar, Bismuth Sulfate Agar) when processing specimens for enteric pathogens. This medium is not recommended for the isolation of Shigella species. PRINCIPLES: The selective agent, brilliant green, is incorporated in the media to inhibit the growth of gram- positive organisms and gram-negative bacilli. Salmonella will appear as red to pink-white opaque colonies surrounded by a brilliant red medium. Lactose or sucrose fermenters produce a yellow- green colony surrounded by a yellow-green zone. Some strains of Proteus may grow on the medium and product red colonies. It should be noted that slow lactose fermenters, Proteus, Citrobacter and Pseudomonas, will grow on Brilliant Green agar and all mimic enteric pathogens. FORMULA: Brilliant Green Agar Ingredient per liter of DI water
Brilliant Green Agar with Sulfadiazine, Novobiocin and/or Nalidixic Acid contains additional antibiotic in concentrations as indicated. PRECAUTIONS: Brilliant Green Agar and Brilliant Green Agar w/Sulfadiazine are for IN VITRO DIAGNOSTIC USE. Brilliant Green Agar w/ Novobiocin and/or Nalidixic Acid are intended for Laboratory Use only. These media support the growth of pathogens and should be handled with caution by adequately trained personnel under the supervision of a microbiologist. Media showing signs of deterioration or contamination must not be used. Media must be brought to room temperature before use. STORAGE: This media is light sensitive. Store at 2-8°C. Do not use beyond its expiration date. PROCEDURE: Before inoculation is performed, the culture medium should be brought to room temperature. Streak plates with moderately heavy inoculum. Incubate plates and inoculated enrichment broths 18-24 hours at 35oC. Examine primary Brilliant Green Agar plates at 18-48 hours and plates subcultured from broths at 18-24 hours for typical Salmonella colonies. Select colonies and perform additional tests as required. Colonies morphology is presumptive aid and does not provide information for speciation. PERFORMANCE CHARACTERISTICS:
Organisms Salmonella typhimurium ATCC 14028 REFERENCES: 1) Federal Register. 1993. Chicken Disease caused by Salmonella enteritidis; proposed rule.
2) Brit. Med. J. Exp. Path., 6:291, 1925. 3) Zeit. Hyg. Infections, 117:26, 1935. 4) Dr. Michael Opitz, Univ. of Maine-Orono, 1990.
FEDERAZIONE SCACCHISTICA ITALIANA REGOLAMENTO FEDERALE ANTIDOPING (in attesa di approvazione da parte del CONI) Testo approvato dal Consiglio Federale FSI del 22-23 settembre 2001 con recepimento del testo del "Regolamento dell'attività antidoping" approvato dal Consiglio Nazionale del CONI con provvedimento n. 1187 del 5/6/2001 Art. 1 - Definizione del doping
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