Microsoft word - microbiology_complete.doc

BL21(DE3)

F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3)
- an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
- Transformed plasmids containing T7 promoter driven expression are repressed until IPTG
induction of T7 RNA polymerase from a lac promoter.

BL21 (DE3) pLysS

F- ompT gal dcm lon hsdSB(rB- mB-) λ(DE3) pLysS(cmR)
- pLys plasmid chloramphenicol resistant; grow with chloramphenicol to retain plasmid
- Chloramphenicol resistant
- The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymerase which
reduces and almost eliminates expression from transformed T7 promoter containing plasmids
when not induced.

DB3.1

F- gyrA462 endA1 glnV44 ∆(sr1-recA) mcrB mrr hsdS20(rB-, mB-) ara14 galK2 lacY1 proA2
rpsL20 xyl5 ∆leu mtl1
- useful for propagating plasmids containing the ccdB gene (gyrA462 ).
- streptomycin resistant
DH5α

F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80lacZ∆M15 ∆(lacZYA-argF)U169,
hsdR17(rK- mK+), λ–
- nalidixic acid resistant

DH10B (Invitrogen)

F- endA1 recA1 galU galK deoR nupG rpsL ∆lacX74 Φ80lacZ∆M15 araD139
∆(ara,leu)7697 mcrA ∆(mrr-hsdRMS-mcrBC) λ-
- suitable for cloning methylated cytosine or adenine containing DNA
- Streptomycin resistant

JM109

endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ ∆(lac-proAB) glnV44 e14- [F' traD36 proAB+
lacIq lacZ∆M15] hsdR17(rK- mK+)
- Partly restriction-deficient; good strain for cloning repetitive DNA (RecA–).
- nalidixic acid resistant

SURE (Stratagene)

endA1 glnV44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5 uvrC e14- ∆(mcrCB-
hsdSMR-mrr)171 F'[ proAB+ lacIq lacZ∆M15 Tn10]
- increased stability for inverted repeats and Z-DNA
- nalidixic acid resistant
- kanamycin resistant
- tetracycline resistant

SURE2 (Stratagene)

endA1 glnV44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5 uvrC e14- ∆(mcrCB-
hsdSMR-mrr)171 F'[ proAB+ lacIq lacZ∆M15 Tn10 Amy CmR]
- increased stability for inverted repeats and Z-DNA
- nalidixic acid resistant
- kanamycin resistant
- tetracycline resistant
- chloramphenicol resistant for < 40 µg/ml, sensitive for > 100 µg/ml

XL1-Blue (Stratagene)

endA1 gyrA96 thi-1 recA1 relA1 lac glnV44 F'[ ::Tn10 proAB+ lacIq ∆lacZM15] hsdR17(rK-
mK+)
- nalidixic acid resistant
- tetracycline resistant
SW102

F- endA1 recA1 ∆galK deoR nupG rpsL ∆lacX74 Φ80lacZ∆M15 araD139 ∆(ara,leu)7697
mcrA ∆(mrr-hsdRMS-mcrBC) [ λcI857 (cro-bioA) <>]
- Streptomycin resistant

Source: http://francois.schweisguth.free.fr/protocols/Bacterial_strains.pdf