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Professor John Greenman
Professor of Microbiology
Research Group
Centre for Research in Biomedicine (CRIB) Research Interests
Growth, ecology & physiology of microbes:biofilm and planktonic continuous cultureIn vitro biofilms: The use of perfusion biofilm as amodel to study oral conditions (caries,periodontal disease) and oral malodour. Thisgeneral theme also supports research in relatedareas such as screening methods for anti-odour orantimicrobial compounds and effects ofexogenous substrates on caries and malodourprocessesOral Malodour (microbial aetiology andtreatment)Odour research: The relationship betweenmicrobial generation rates in situ, partitioning ofVC's between gas and liquid phase, sensitivityand specificity of detector system (including thehuman nose) and finally "the perception" andother effects of smell on the perceiver.
Role of oral and skin microbes in health anddisease and microbiology of exfoliative surfaces.
As part of this, interests include methods fortesting antimicrobial efficacy of wound dressingsor other active surfaces, antimicrobialphotodynamic treatment, effects of antimicrobialcompounds (including ECAS) on bacteria, and invitro models.
Constantly monitored in vitro biofilm models Research Supervisions
2013 GABA International AG – all rights reserved Director of Studies
2nd Supervisor
Professional Memberships
Society for General MicrobiologyInternational Assocation of Dental ResearchBritish Society for Dental ResearchOral Microbiology & Immunology GroupAssociate of the Royal College of PathologistsPresident - International Society for Breath Odor Research (ISBOR) 2007-2009 External Committees
ISBOR Conference organizer and chairman, London MeetingISBOR Conference Proceedings editor, London MeetingISBOR Conference Editorial Board, Chicago MeetingMember of the Advisory Editorial Board of Oral Diseases Qualifications
1972 BSc General Degree in Life Sciences, University of Liverpool1973 BSc Honours Degree, 2(i) Microbiology, University of Liverpool1976 PhD Research Degree, Oral Microbiology, University of Liverpool Reviewing
1997 to 1998 National Health Service Member of peer-review panel, NHS National R&D programme in primary dental care QAA SPR reviewer in Molecular/Organismal Biosciences Journal Referee:Microbial Ecology in Health and Disease,Archives of Oral Biology, Microbiology, Anaerobe, Journalof Dental Research, Enzyme & Microbial Technology (vii.)Journal of Photochemistry and Photobiology B: Biology(viii.) Gerodontology Grant Referee: Medical Research Council (MRC), TheWelcome Trust, Agricultural and Food Research Council(AFRC), Biotechnology & Biological Sciences ResearchCouncil (BBSRC), British Diabetic Association 2013 GABA International AG – all rights reserved 2000 to 2007 Journal Referee: Journal Applied Microbiol, Archives of Oral Biology, Microbiology, Anaerobe, Journal of DentalResearch, Enzyme & Microbial Technology, Journal ofPhotochemistry and Photobiology B: Biology (viii.)Gerodontology, Oral Diseases 2000 to 2007 Grant Referee: Medical Research Council (MRC), The Welcome Trust, Agricultural and Food Research Council(AFRC), Biotechnology & Biological Sciences ResearchCouncil (BBSRC), British Diabetic Association, SkinResearch Foundation, Wellcome Trust.
Teaching
BSc (Hons) Applied Biological Sciences (FT & PT)BSc (Hons) Biomedical Sciences (FT and Block Release PT)BSc Biomedical Sciences (PT)BSc (Hons) Medical Microbiology (PT)Antimicrobial Agents (L3); Microbial Ecology; Medical microbiology; Oral & Dentalbacteriology; Anaerobic microbiology; Continuous culture techniques (chemostats andbiofilms); microbial physiology and nutrition; Perfusion biofilms for testing antimicrobialcompoundsMicrobial Fuel Cells and Ecobot;Contribute to BSc ABS, MSc Med Bact and higher degree programs Recent Publications
Hess, J., Greenman, J. and Duffield, J.R. (2008), Modelling oral malodour from a tonguebiofilm, Journal of Breath Research, 2: 017003 (6pp) doi: 10.1088/1752-7155/2/1/017003J Greenman, C McKenzie, S Saad, B Wiegand and JC Zguris (2008), Effects of chlorhexidineon a tongue-flora microcosm and VSC production using an in vitro biofilm perfusion model,Journal of Breath Research, 2 046005 (7pp) doi: 10.1088/1752-7155/2/4/046005Scully, C. and Greenman, J. (2008), Halitosis (breath odor), Periodontology 2000, 48: 66-75Spencer P, Greenman J, McKenzie C, Gafan G, Spratt D and Flanagan A. (2007), In vitrobiofilm model for studying tongue flora and malodour, Journal of Applied Microbiology,103: 985-992.
Thorn, R.M.S, Nelson, S.M., and Greenman, J. (2007), Use of a BioluminescentPseudomonas aeruginosa Strain within an In Vitro Microbiological System, as a Model ofWound Infection, To Assess the Antimicrobial Efficacy of Wound Dressings by MonitoringLight Production, Antimicrobial Agents and Chemotherapy, ISSN: 0066-4804, 3217-3224.
Greenman J, Thorn RMS, Saad S and Austin AJ. (2006), In vitro diffusion bed, 3-day repeatchallenge 'capacity' test for antimicrobial wound dressings, International Wound Journal, 3:322-329Thorn RMS, J. Greenman, A. Austin (2006), An in vitro study of antimicrobial activity and 2013 GABA International AG – all rights reserved efficacy of iodine-generating hydrogel dressings, Journal of Wound Care, 15: 305-310Thorn RMS, J. Greenman and A.J. Austin. (2005), ‘In vitro method to assess theantimicrobial activity and potential efficacy of novel types of wound dressings’, Journal ofAppl. Microbiol, 99: 895-901.
Greenman, J., El-Maaytah, M., Duffield, J., Spencer, P., Rosenberg, M., Corry, D., Saad, S.,Lenton, P., Majerus, G., and Nachnani, S. (2005), Assessing the relationship betweenconcentrations of malodor compounds and odor scores from judges, JADA, 136: 749-757Greenman, J., Spencer, P., McKenzie, C., Saad, S. & Duffield, J. (2005), Review: In vitromodels for oral malodour., Oral Diseases, 11 (suppl.1): 14-23Saad, S., Greenman, J., Duffield, J., Sudlow, K. (2005), Use of n-butanol as an odourant tostandardize the organoleptic scale of breath odour judges, Oral Diseases 11 (suppl.1):45-47.
Marques, C.N.H., Salisbury, V.C., Greenman, J., Bowker, K.E. and nelson, S.M. (2005),Discrepancy between viable counts and light output as viability measurements, followingciprofloxacin challenge of self-bioluminescent Pseudomonas aeruginosa biofilms, Journal ofAntimicrobial Chemotherapy 56: 665-671.
Greenman, J., Duffield, J., Spencer, P., Rosenberg, M., Corry, D., Saad, S., Lenton, P.,Majerus, G., Nachnani, S. and El-Maaytah, M. (2004), Study on the organoleptic intensityscale for measuring oral malodour, Journal of Dental Research 83: 81-85.
Zeina, B., Greenman, J., Corry, D., & Purcell, W.M. (2003), Antimicrobial photodynamictherapy: assessment of genotoxic effects on keratinocytes in vitro., Br J Dermatol 148,Pages: 229-232.
University of the West of EnglandFrenchay CampusBristol, BS16 1QYTelephone: +44(0)117 32 82515E-mail: [email protected] Number: 2G1A 2013 GABA International AG – all rights reserved

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