Microsoft word - illumina sequence information for customers dec2008.doc
Dear Customer, This communication is in response to your request for particular oligonucleotide sequences for use with the Illumina Genome Analyzer and associated assays. Below please find the oligonucleotide sequences that we can make available to you. This communication is solely for your use and should not be distributed outside your institution. The oligonucleotide sequences are protected by copyright which is owned by Illumina. Illumina allows you to reproduce the oligonucleotide sequences for use with the Illumina Genome Analyzer and associated assays. Additionally, Illumina realizes customers may need to make alterations to the oligonucleotide sequences that are necessary to allow use with the Illumina Genome Analyzer and associated assays. Thus, Illumina allows you to make such necessary alterations to the oligonucleotide sequences but only for use with the Illumina Genome Analyzer and associated assays. Illumina grants you no other rights to use, reproduce or otherwise disclose the oligonucleotide sequences. Alteration or modification of the oligonucleotide sequences for use with non-Illumina products is not allowed. If you reproduce the oligonucleotide sequences for viewing within your institution, the following copyright notice must remain affixed to the sequences:
Oligonucleotide sequences 2006-2008 Illumina, Inc. All rights reserved. Illumina customers may reproduce and create derivative works of the oligonucleotide sequences but only for use with the Illumina Genome Analyzer and associated assays. All other uses are strictly prohibited.
If you reproduce these oligonucleotide sequences for viewing outside your institution (e.g. journal publication), you must affix the following copyright notice to the sequences:
Oligonucleotide sequences 2006-2008 Illumina, Inc. All rights reserved.
Genomic DNA oligonucleotide sequences Adapters1 PCR Primers1
5' AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
Genomic DNA Sequencing Primer 5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT
1 The genomic DNA adapters and PCR primers are additionally modified, treated and purified in a proprietary manner. We cannot guarantee efficient cluster generation or sequencing if customer does not use adapters and PCR primers purchased from Illumina in the genomic DNA sample prep kit.
Multiplexing DNA oligonucleotide sequences Multiplexing Adapters1 Multiplexing PCR Primer 1.01
5' AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
Multiplexing PCR Primer 2.01 5' GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT Multiplexing Read 1 Sequencing Primer 5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT Multiplexing Index Read Sequencing Primer 5' GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Multiplexing Read 2 Sequencing Primer 5' GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT PCR Primer Index 1 CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTC PCR Primer Index 2 CAAGCAGAAGACGGCATACGAGATACATCGGTGACTGGAGTTC PCR Primer Index 3 CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTC PCR Primer Index 4 CAAGCAGAAGACGGCATACGAGATTGGTCAGTGACTGGAGTTC PCR Primer Index 5 CAAGCAGAAGACGGCATACGAGATCACTGTGTGACTGGAGTTC PCR Primer Index 6 CAAGCAGAAGACGGCATACGAGATATTGGCGTGACTGGAGTTC PCR Primer Index 7 CAAGCAGAAGACGGCATACGAGATGATCTGGTGACTGGAGTTC PCR Primer Index 8 CAAGCAGAAGACGGCATACGAGATTCAAGTGTGACTGGAGTTC PCR Primer Index 9 CAAGCAGAAGACGGCATACGAGATCTGATCGTGACTGGAGTTC PCR Primer Index 10 CAAGCAGAAGACGGCATACGAGATAAGCTAGTGACTGGAGTTC PCR Primer Index 11 CAAGCAGAAGACGGCATACGAGATGTAGCCGTGACTGGAGTTC PCR Primer Index 12 CAAGCAGAAGACGGCATACGAGATTACAAGGTGACTGGAGTTC
Paired End DNA oligonucleotide sequences PE Adapters1 5' P-GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG PE PCR Primer 1.01 5' AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT PE PCR Primer 2.01 5' CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT PE Read 1 Sequencing Primer PE Read 2 Sequencing Primer 5' CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT
DpnII gene expression oligonucleotide sequences Gex Adapter 1 5' P-GATCGTCGGACTGTAGAACTCTGAAC Gex Adapter 2 Gex PCR Primer 1 5' CAAGCAGAAGACGGCATACGA Gex PCR Primer 2 5' AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA Gex Sequencing Primer
NlaIII gene expression oligonucleotide sequences Gex Adapter 1 Gex Adapter 2 Gex PCR Primer 1 5' CAAGCAGAAGACGGCATACGA Gex PCR Primer 2 5' AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA Gex Sequencing Primer
Small RNA oligonucleotide sequences RT Primer 5' RNA Adapter 3' RNA Adapter Small RNA PCR Primer 1 5' CAAGCAGAAGACGGCATACGA Small RNA PCR Primer 2 5' AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA Small RNA Sequencing Primer
Please contact us if you have any questions. Sincerely, Customer Support - Illumina Inc. 858-202-4566
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Dear Customer, This communication is in response to your request for particular oligonucleotide sequences for use with the Illumina Genome Analyzer and associated assays. Below please find the oligonucleotide sequences that we can make available to you. This communication is solely for your use and should not be distributed outside your institution. The oligonucleotide sequences are protected by copyright which is owned by Illumina. Illumina allows you to reproduce the oligonucleotide sequences for use with the Illumina Genome Analyzer and associated assays. Additionally, Illumina realizes customers may need to make alterations to the oligonucleotide sequences that are necessary to allow use with the Illumina Genome Analyzer and associated assays. Thus, Illumina allows you to make such necessary alterations to the oligonucleotide sequences but only for use with the Illumina Genome Analyzer and associated assays. Illumina grants you no other rights to use, reproduce or otherwise disclose the oligonucleotide sequences. Alteration or modification of the oligonucleotide sequences for use with non-Illumina products is not allowed. If you reproduce the oligonucleotide sequences for viewing within your institution, the following copyright notice must remain affixed to the sequences:
Oligonucleotide sequences 2006-2008 Illumina, Inc. All rights reserved. Illumina customers may reproduce and create derivative works of the oligonucleotide sequences but only for use with the Illumina Genome Analyzer and associated assays. All other uses are strictly prohibited.
If you reproduce these oligonucleotide sequences for viewing outside your institution (e.g. journal publication), you must affix the following copyright notice to the sequences:
Oligonucleotide sequences 2006-2008 Illumina, Inc. All rights reserved.