Helicobacter clarithromycin resistant (Yellow) Helicobacter clarithromycin sensitive (Green)
HELICOBACTER PYLORI AND MACROLIDE RESISTANCE
Rapid Identification from Biopsy Material (frozen and
formalin fixed, paraffin-embedded sections)
A One Hour Procedure Utilising Specific DNA Probes
1 Admiralty Way Camberley Surrey GU15 3DT United Kingdom
Phone: +44 1276 600081 Fax: +44 1276 600151 E-mail: [email protected]
The recognition of the association between H. FISH is, pylori infection and peptic ulcers was a major
breakthrough in gastroenterology. Reports •
suggest that peptic ulcer is rare without either H.
pylori or NSAID’s. It is present in almost all cases •
Not susceptible to interference from other
of duodenal ulcer and most cases of gastric ulcer.
Histopathology is a sensitive, rapid and easy •
method for diagnosis of H. pylori infection,
however it does not provide information about
the resistance pattern. Although culturing of H. •
pylori enables the antimicrobial sensitivity pattern
fixed, paraffin-embedded sections or direct
of the organism to be determined, it is not widely
used in routine laboratories because H. Pylori
grows very slowly and requires strict conditions.
Non-invasive clinical tests like the Urea breath
test and serological methods are well established
screening procedures which help to reduce the •
Mixed populations of resistant and sensitive
cost and workload of invasive endoscopy.
However antimicrobial susceptibility testing of H.
pylori obtained from gastric biopsies is essential •
prior to the first therapy and after the first
treatment failure, since Macrolide resistance in
this pathogen is considered a main reason for BACTfish™ kits employ a patented procedure,
failure of antibiotic eradication therapy. whereby the sample slides are fixed in a
Unfortunately, phenotypic resistance testing in BACTwave™; a microwave based thermocycler,
vitro is usually not available until 48—96 hours before treatment. Hybridisation is carried out in
the BACTwave™ thermocycler, cycling between
46°C and 48°C. The cycling induces touch-down
Resistance to Clarithromycin always correlates annealing which decreases turnaround time to 60
with a significant decrease in therapeutic efficiency minutes.
predominantly by distinct point mutations within Product Details:
the peptidyltransferase region of the 23S rRNA.
Fluorescence In Situ Hybridisation (FISH) is a
powerful technique that can be used to determine
the presence of Helicobacter pylori in frozen or
formalin-fixed, paraffin-embedded sections.
Positive results from sections up to four years old
have been reported. In addition FISH can also
determine the resistance status to macrolides BACTwave™ Thermal Cycler
from the same sample and at the same time.
FISH can detect mixed populations of sensitive
and resistant organisms and as it is based on A full catalogue of support reagents and materials
whole cell hybridisation, maintains the cell is available upon request. DNA probes are
structure for morphological studies. H. pylori can available as tandems or singly to allow method
form coccoid, non-cultivable forms in vivo. FISH is customisation.
able to identify these forms with the same
Source: http://www.microgenbioproducts.com/pdf/Product%20Brochures/BACTfish_Pylori.pdf
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Springtime outdoor safety Whether you're relaxing in the backyard, turning up your garden, hitting the pool, or exploring the great outdoors, here are some ways to help keep you and your family healthy this spring and summer: • Beware of bugs: mosquitoes, ticks, and other i