Andrea R. Castillo, Assistant Professor Curriculum Vitae Eastern Washington University 258 Science Building Cheney, WA 99004-2440 Phone: (509) 359-2866, Fax: (509) 359-6867, email: [email protected]
Current Position: Assistant Professor of Biology, Eastern Washington University
Postdoctoral fellow with Dr. Karen Ottemann, University of California, Santa Cruz, CA
Postdoctoral fellow with Dr. Sue Biggins, Fred Hutchinson Cancer Research Center, Seattle, WA
Ph.D. in Molecular, Cellular and Developmental Biology; Spindle pole body duplication in
Sacchormyces cerevisiae, University of Colorado, Boulder, CO
B.S. in Biology; Albertson College of Idaho, Caldwell, ID
Postdoctoral research experience: Adapted and used an in vivo expression system (RIVET) to identify Helicobacter pylori genes
induced in response to host tissue in the lab of Dr. Karen Ottemann at the University of California, Santa Cruz. when: 2003-present
Used the conditional centromere in Saccharomyces cerevisiae to determine cell cycle requirements
for kinetochore assembly in the lab of Dr. Sue Biggins at Fred Hutchinson Cancer Research Center. when: 2001-2003
Doctoral research experience: Identified MPS1 separation of function alleles and characterized their role in spindle pole
body duplication in the lab of Dr. Mark Winey at the University of Colorado, Boulder. when: 1997-2001
Mapped the Arabidopsis thaliana accelerated cell death mutant, acd2 in the lab of Dr. Jean
Greenberg at the University of Colorado, Boulder. when: 1995-1997
Undergraduate research experience: Tested the feasibility of using transposon tagging to clone mutator suppressible mutants in
maize with Dr. Robert Martienssen at Cold Spring Harbor Laboratory, NY. when: 1993
Determined the evolutionary relationship between six populations of Primula cusickiana
using restriction fragment length polymorphism analysis with Dr. Art Ayers at Albertson College. Andrea Castillo, Ph.D. curriculum vitae
Guest lecturer, University of California, Santa Cruz, CA
subject: Microbiology, pathogenesisclass size: ~180 students
Supervising undergraduate research projects, University of
subject: Helicobacter pylori pathogenesis and transcriptional terminationproject duration: 1-2 years
Minority Arts and Science Instructor, University of Colorado, Boulder, CO
subjects: molecular biology, geneticsclass size: 7 students
Teaching Assistant for undergraduate courses, University of Colorado,
Boulder, COsubjects: molecular biology, cell biologyclass size: ~20 students
Co-written with Karen Ottemann,Ph.D., Associate Professor, University of California, Santa Cruz, CA
Fred Hutchinson Cancer Research Center, Seattle, WA
Bachelor of Science, summa cum laude
Cold Spring Harbor Undergraduate Fellowship
*Castillo, A.R., A. Woodruff, E. Cabral, L. Connolly, K.M. Ottemann. In Press. RIVET
identifies novel factors required for Helicobacter pylori host colonization. Infection and Immunity. Castillo, A.R., S.A. Arevalo, A. Woodruff, K.M. Ottemann. 2008. Experimental analysis
of Helicobacter pylori transcriptional terminators suggests this microbe uses both intrinsic and factor-dependent termination. Mol. Micro., 67(1) :155-170
Collins, K.A., A.R. Castillo, S.Y. Tatsutani, S. Biggins. 2005. De novo kinetochore assembly
requires the centromeric histone H3 variant. Mol. Biol. Cell, 16(12): 5649-60. Castillo, A.R., J.B. Meehl, G. Morgan, A.R. Schutz, M. Winey. 2002. The Yeast Protein Kinase
Mps1p is Required for Assembly of the Integral Spindle Pole Body Component Spc42p. J. Cell Biol., 156(3): 453-65.
Mach, J.M., A.R. Castillo, R. Hoogstraten, J.T. Greenberg,. 2001. The Arabidopsis-accelerated
cell death gene ACD2 encodes red chlorophyll catabolite reductase and suppresses the spread of disease symptoms. Proc. Natl. Acad. Sci. USA, 98(2): 771-776. Andrea Castillo, Ph.D. curriculum vitae
Jones, M.H., J.B. Bachant, A.R. Castillo, T.H. Giddings, Jr., M. Winey. 1999.
Yeast Dam1p is required to maintain spindle integrity during mitosis and interacts with the Mps1p kinase. Mol. Bio. Cell, 10: 2377-2391. Poster and Oral Presentation: Castillo, A.R., S.A. Arevalo, A. Woodruff, K.M. Ottemann. 2007. Experimental analysis of Helicobacter pylori transcriptional terminators suggests this microbe uses both intrinsic and factor dependent termination. Speaker at the West Coast Bacterial Physiology Meeting, Pacific Grove, CA. Castillo, A.R., A. Woodruff, E. Cabral, L. Connolly, K.M. Ottemann. 2007. Use of RIVET in Helicobacter pylori identifies novel putative virulence factors. Poster at the Microbial Pathogenesis and Host Response Meeting, Cold Spring Harbor, NY. Castillo, A.R., A. Woodruff, E. Cabral, L. Connolly, K.M. Ottemann. 2006. Using RIVET to identify Helicobacter pylori virulence factors. Speaker at the Bay Area Pathogenesis Meeting, San Francisco, CA. Castillo, A.R., A. Woodruff, E. Cabral, L. Connolly, K.M. Ottemann. 2005. Using RIVET to identify Helicobacter pylori virulence factors. Speaker at the West Coast Bacterial Physiology Meeting, Pacific Grove, CA. Castillo, A.R., A. Woodruff, E. Cabral, L. Connolly, K.M. Ottemann. 2005. Using RIVET to identify Helicobacter pylori virulence factors. Poster at the Microbial Pathogenesis and Host Response Meeting, Cold Spring Harbor, NY. Castillo, A.R. and S. Biggins. 2002. Regulation of Kinetochore Assembly. Poster at the Yeast Chromosome
Structure and Segregation FASEB meeting, Snowmass, CO. Castillo, A.R. and M. Winey. 1998. Defining MPS1 function in spindle pole body duplication and the spindle
assembly checkpoint through isolation of new mps1 alleles and genes that genetically interact with MPS1.Poster at theYeast Chromosome Structure and Segregation FASEB meeting, Snowmass, CO. Castillo, A.R. and J.T. Greenberg. 1996. Acd2: A potential negative regulator of the hypersensitive response
in Arabidopsis thaliana.Poster at the Plant Senescence and Cell Death Gordon Conference, Plymouth, NH. Other Activities and Honors:
American Society for Microbiology Kadner Institute Attendee
Organized CU-Boulder graduate student symposium
Postdoctoral Advisor Postdoctoral Advisor
Karen Ottemann, Ph.D., Associate Professor
Doctoral Thesis Advisor Mark Winey, Ph.D., Professor University of Colorado, Boulder, MCD Biology Boulder, CO 80309 Phone: (303) 492-3409, e-mail:
Prozac and the New Antidepressants, William Appleton, Pearson Education, Limited, 2002, , . . The Antidepressant Fact Book What Your Doctor Won't Tell You about Prozac, Zoloft, Paxil, Celexa,and Luvox, Peter Roger Breggin, 2001, Medical, 226 pages. Discusses the meaning of depressionand the many and various impacts on the brain of SSRI antidepressants, including their dangers,side effects, and th
MEDICAL MISSION TRIP 2006 2006 Randolph World Ministries, Inc. Team Members Accomplishments of the 2006 Medical Mission Trip Through the generous contributions of my laboratory partners I was able to deliver 21 boxes of laboratory supplies weighing 255 pounds. Most of these supplies will be used in the laboratory at Bethesda Medical Center, our main Haitian clinic. The remaini